CST, 55216S, TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying TCRdelta. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #55216) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein. Background The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4). Alternate Names B7Z8K6; delta-chain (C-delta region); T cell receptor delta constant; T-cell receptor delta chain C region; TCRD; TRDC Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG