CST, 55222S, RAGE (E7I6S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAGE. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RAGE (E7I6S) Rabbit Monoclonal Antibody (CST #55222) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RAGE (E7I6S) Rabbit Monoclonal Antibody detects endogenous levels of total RAGE protein. This antibody detects isoform 1 of human RAGE, and is predicted to detect multiple RAGE isoforms in mouse, including xRAGE, mRAGE, and sRAGE. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human RAGE protein. Background The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin (Ig) superfamily. It can be expressed as full-length, membrane-bound RAGE isoform 1 or as a secreted sRAGE protein that lacks a transmembrane domain (1). RAGE is detected during early developmental stages and in the lung under normal physiological conditions (2), and it is upregulated at sites of inflammation (3). Advanced glycation end products (AGEs) and a variety of other ligands interact with this receptor (1). Ligand binding activates full-length RAGE and initiates downstream signaling pathways that include activation of NF-κB, which leads to production of pro-inflammatory cytokines and inflammation (4). Activation of these pathways has been implicated in various disease states including Alzheimer's disease, diabetes, arthritis, and atherosclerosis (4). Soluble RAGE can competitively bind RAGE ligands in the extracellular environment, which prevents ligand interaction with full-length RAGE at the cell surface (1). Alternate Names Advanced glycosylation end product-specific receptor; advanced glycosylation end-product specific receptor; AGER; MGC22357; RAGE; receptor for advanced glycation end-products variant 20; receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 48, 52, 58 Source/Isotype: Rabbit IgG