CST, 55269SF, IDH1 (D2H1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IDH1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IDH1 (D2H1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #55269) is ready to ship. Product Usage Information This product is the carrier free version of product #8137. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8137 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IDH1 (D2H1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IDH1 protein. This antibody does not recognize endogenous IDH2 protein, but does recognize IDH2 when recombinantly overexpresssed. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg222 of human IDH1 protein. Background IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP respectively, as an electron acceptor (1). IDH1 is the NADP -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochondrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Mutations in IDH1 have been reported in glioblastoma (4), acute myeloid leukemia (5,6), and other malignancies (7). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (8). Alternate Names Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDHC; IDP; IDPC; isocitrate dehydrogenase (NADP(+)) 1; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; Isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+), soluble; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; NADP(+)-specific ICDH; NADP+-specific ICDH; Oxalosuccinate decarboxylase; PICD Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG