CST, 55284S, NRCAM Antibody

Polyclonal Antibody for studying NRCAM. Validated for Western Blotting. Highly specific and rigorously validated in-house, NRCAM Antibody (CST #55284) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody Protocol Available protocols: Western Blotting Specificity / Sensitivity NRCAM Antibody recognizes endogenous levels of NRCAM protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NRCAM protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Neuronal Cell Adhesion Molecule, or NRCAM, belongs to the immunoglobulins Cell Adhesion Molecules (CAM's) superfamily (1). NRCAM, an ankyrin-binding protein, contributes to the neurite outgrowth by providing directional signaling during axonal cone growth (2, 3, 4). Additionally, it plays a role in mediating the interaction between axons and Schwann cells and contributes to the formation and maintenance of Nodes of Ranvier (5, 6, 7, 8). NRCAM also plays an important role in the establishment of dendritic spines in developing cortical neurons (9). NRCAM is expressed in non-neuronal cells, mostly in endothelial cells (10). Alternate Names hBravo; KIAA0343; MGC138845; MGC138846; Neuronal cell adhesion molecule; Neuronal surface protein Bravo; Ng-CAM-related; NgCAM-related cell adhesion molecule; Nr-CAM; NRCAM; NrCAM bravo Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit