CST, 55361T, Numb (F4G6B) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Numb. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Numb (F4G6B) Mouse Monoclonal Antibody (CST #55361) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Numb (F4G6B) Mouse Monoclonal Antibody recognizes endogenous levels of total Numb protein. This antibody detects a 100 kDa band of unknown origin in some samples. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala486 of human Numb protein. Background Numb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9). Alternate Names c14_5527; C14orf41; h-Numb; NUMB; NUMB endocytic adaptor protein; numb homolog; numb homolog (Drosophila); Protein numb homolog; Protein S171; S171 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72, 74 Source/Isotype: Mouse IgG1 kappa