CST, 5536T, Phospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for WB,WB,WB,IP,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody (CST #5536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78080 . Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR protein. Background The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). Alternate Names FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin kinase; mTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; SKS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 289 Source/Isotype: Rabbit IgG