CST, 55421W2, EpCAM (VU1D9) Mouse Monoclonal Antibody (SignalFlex™ mFluor™ UV375 Conjugate)

Monoclonal Antibody for studying EPCAM. Highly specific and rigorously validated in-house, EpCAM (VU1D9) Mouse Monoclonal Antibody (SignalFlex^™ mFluor^™ UV375 Conjugate) (CST #55421) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity EpCAM (VU1D9) Mouse mAb (SignalFlex™ mFluor™ UV375 Conjugate) detects endogenous levels of total EpCAM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells. Background Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3). Alternate Names ACSTD1; Adenocarcinoma-associated antigen; carcinoma-associated antigen GA733-2; CD326; Cell surface glycoprotein Trop-1; CO-17A; CO17-1A; DIAR5; EGP; EGP-2; EGP314; EGP34; EGP40; Ep-CAM; EPCAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; hEGP-2; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; M1S2; M4S1; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACST-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; Tumor-associated calcium signal transducer 1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1