CST, 5543S, Phospho-PZR (Tyr263) Antibody

Polyclonal Antibody for studying PZR (Tyr263) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-PZR (Tyr263) Antibody (CST #5543) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-PZR (Tyr263) Antibody recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263. This antibody may cross-react with other activated receptor tyrosine kinases Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8). Alternate Names FLJ21047; immunoglobulin family transmembrane protein; MPZL1; MPZL1b; myelin protein zero like 1; myelin protein zero-like 1; Myelin protein zero-like protein 1; protein zero related; Protein zero-related; PZR; PZR1b; PZRa; PZRb Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 30-50 SOURCE: Rabbit