CST, 5544S, TGF-beta Receptor III (D11G10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TGFBR3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TGF-beta Receptor III (D11G10) Rabbit Monoclonal Antibody (CST #5544) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TGF-beta Receptor III (D11G10) Rabbit Monoclonal Antibody detects endogenous levels of total TGF-β Receptor III protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of TGF-β receptor III protein. Background Transforming growth factor-β (TGF-β) proteins belong to the TGF-β superfamily of cytokines that play a critical role in regulating cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-3). TGF-β ligands elicit signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII) TGF-β receptors. Type I and type II receptors are serine/threonine kinases that form a heteromeric complex following ligand binding to the type II receptor. In response to ligand binding, the type II receptors form a stable complex with the type I receptors, triggering phosphorylation and activation of the type I receptor (4). This results in the recruitment of receptor-mediated SMADs (SMAD2, SMAD3), which are phosphorylated by the type I kinase in an SSXS domain in the C-terminus. This leads to recruitment of the co-SMAD (SMAD4), and subsequent translocation of this heteromeric SMAD complex to the nucleus, where it regulates transcription of target genes (5-7). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex (8). Alternate Names Betaglycan; betaglycan proteoglycan; BGCAN; TGBR3; TGF-beta receptor type 3; TGF-beta receptor type III; TGFBR3; TGFR-3; transforming growth factor beta receptor 3; Transforming growth factor beta receptor III; Transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG