CST, 55481T, ALDH1A2 (E6O6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ALDH1A2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, ALDH1A2 (E6O6Q) Rabbit Monoclonal Antibody (CST #55481) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ALDH1A2 (E6O6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALDH1A2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro9 of human ALDH1A2 protein. Background The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6). Alternate Names AL1A2; aldehyde dehydrogenase 1 family member A2; aldehyde dehydrogenase 1 family, member A2; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; MGC26444; RALDH 2; RALDH(II); RALDH2; RALDH2-T; Retinal dehydrogenase 2; Retinaldehyde-specific dehydrogenase type 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG