CST, 5548S, S6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying S6 Ribosomal Protein. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, S6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #5548) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity S6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein. Background One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5). Alternate Names 40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1