CST, 55494T, GFP (5G4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GFP jellyfish. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GFP (5G4) Mouse Monoclonal Antibody (CST #55494) is ready to ship. Product Usage Information Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GFP (5G4) Mouse Monoclonal Antibody detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody does not detect RFP-tagged proteins. Please note that the GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with native GFP. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1). Alternate Names GFP; Green fluorescent protein Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2b