CST, 55546T, FcRn (E9J8W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcRn. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, FcRn (E9J8W) Rabbit Monoclonal Antibody (CST #55546) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33461 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity FcRn (E9J8W) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcRn protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly250 of human FcRn protein. Background The neonatal Fc receptor, FcRn, is an atypical Fc gamma receptor that bears homology to class I MHC (major histocompatibility complex) and associates with β2-microglobulin (1). FcRn is expressed by the vascular endothelium, several epithelial cell types, and hematopoietic cells including several innate immune cell types and B cells (2). FcRn mediates recycling and transcytosis of IgG, resulting in protection of IgG from catabolism and an extended serum half-life of IgG relative to other antibody isotypes (3). In addition, FcRn transports IgG across the placenta and also mediates uptake of maternal IgG from milk, resulting in enhanced humoral immunity in the fetus and neonates (1,4,5). FcRn also plays an important role in initiation of adaptive immunity by transporting immune complexes to intracellular dendritic cell compartments for antigen processing and presentation to T cells (6-8). In addition to IgG, FcRn also binds albumin and similarly protects it from catabolism, leading to extended half-life and high plasma concentrations (9). Alternate Names alpha-chain; Fc fragment of IgG receptor and transporter; Fc fragment of IgG, receptor, transporter, alpha; FCGRN; FCGRT; FCRN; FcRn alpha chain; heavy chain of the major histocompatibility complex class I-like Fc receptor; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; Neonatal Fc receptor; neonatal Fc-receptor for Ig; transmembrane alpha chain of the neonatal receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG