CST, 55550T, Histamine H1 Receptor (F5Q1L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying H1R. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Histamine H1 Receptor (F5Q1L) Rabbit Monoclonal Antibody (CST #55550) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Histamine H1 Receptor (F5Q1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total H1R protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys332 of human H1R protein. Background Histamine is a biogenic amine that is involved in a range of physiological responses and signaling events through binding to histamine receptors (1,2). Histamine was initially discovered as a key molecule involved in allergic response and anaphylaxis, and the use of antihistamines for allergic disorders can be traced back to the 1950s (1,4). Histamine mediates its effects through a family of G-protein coupled receptors, each characterized by a specific pattern of cellular expression and functions. Most allergic and inflammatory actions of histamine are mediated by the type 1 histamine receptor (H1R) (1-4). The H1R protein contains seven transmembrane domains and an extracellular amino-terminal glycosylated domain (2). Binding of histamine to H1R results in signaling cascades for notable pathways such as phospholipase C, calcium ion transport, protein kinase C (PKC) activation, and NF-κB signaling (3,4). H1R activation leads to increased chemotaxis of inflammatory eosinophils and neutrophils, increased activity of antigen-presenting cells (APCs), activation of T cells, decreased humoral immunity, and increased IgE production (1,3,4). H1R is expressed in a diverse group of cell types such as neurons, endothelial cells, muscle, hepatocytes, and chondrocytes, in addition to key immune cell types such as monocytes, neutrophils, eosinophils, dendritic cells (DCs), T cells, and B cells (3,4). H1R knockout mice show reduced T cell response and decreased inflammatory response (4). By analyzing publicly available RNA-seq databases (5), the co-expression and interaction of CXCR4 and HRH1 are implicated in lower survival in breast cancer, and dysregulated H1R expression was observed as an early event in the onset of malignant transformation of some cancer cell types (5-8). Recently, it has been observed that H1R promotes cell proliferation in several cancer types, including brain, pancreatic, and breast cancers, underscoring that H1R is a potential target in cancer therapy (5-8). Alternate Names H1-R; H1R; HH1R; hisH1; histamine H(1) receptor; Histamine H1 receptor; histamine receptor H1; histamine receptor, subclass H1; HRH1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG