CST, 5555T, DDX4 (2F9H5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying DDX4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX4 (2F9H5) Mouse Monoclonal Antibody (CST #5555) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DDX4 (2F9H5) Mouse Monoclonal Antibody detects endogenous levels of total DDX4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human DDX4. Background DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1). Alternate Names DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; DEAD-box helicase 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Mouse IgG1