CST, 5556S, DLST Antibody

Polyclonal Antibody for studying DLST. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DLST Antibody (CST #5556) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DLST Antibody detects endogenous levels of total DLST protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro188 of human DLST protein. Antibodies are purified by protein A and peptide affinity chromatography. Background α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimer's disease (2). Alternate Names 2-oxoglutarate dehydrogenase complex component E2; dihydrolipoamide S-succinyltransferase; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; DLST; DLTS; E2K; KGD2; ODO2; OGDC-E2; PGL7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit