CST, 55584S, CD27 (O323) Mouse Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying CD27. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD27 (O323) Mouse Monoclonal Antibody (PE Conjugate) (CST #55584) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:20 Flow Cytometry (Live): 1:20 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD27 (O323) Mouse Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD27 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Human Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells, and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR-associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface, and soluble CD27 is used as a marker of T cell activation (7,8). This O323 antibody is used for flow cytometric analysis of CD27 expression on various cell populations. Alternate Names CD27; CD27 antigen; CD27 molecule; CD27L receptor; MGC20393; S152; S152. LPFS2; T cell activation antigen CD27; T cell activation antigen S152; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; Tp55; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa