CST, 55653S, C1R (E3R7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying C1R. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, C1R (E3R7T) Rabbit Monoclonal Antibody (CST #55653) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity C1R (E3R7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total C1R protein. This antibody reacts with C1R proenzyme and C1R A (heavy) chain proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human C1R protein. Background Complement C1R subcomponent (C1R) is a secreted serine protease module of the C1 macromolecular complex, which is a central node in complement activation via the classical pathway. Each C1 complex is stabilized by divalent calcium cations and contains, in part, a non-covalent dimer of identical C1R monomers that interact with the C1Q component of the C1 macromolecular complex (1). Each C1R monomer is synthesized as a proenzyme and is auto-activated within the C1 complex upon recognition of antigen-antibody complexes by C1Q (2,3). Activation of C1R leads to its cleavage into a disulfide-linked amino-terminal A (heavy) chain and a carboxyl terminal B (light) chain. The B chain contains serine protease activity that triggers a cascade of downstream enzymatic reactions that govern the classical pathway of complement (4,5). Alternate Names C1R; complement C1r; Complement C1r subcomponent; Complement C1r subcomponent heavy chain; Complement C1r subcomponent light chain; Complement component 1 subcomponent r; complement component 1, r subcomponent; EDSPD1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95,55 Source/Isotype: Rabbit IgG