CST, 55713S, CD44 (E7K2Y) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying CD44. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD44 (E7K2Y) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #55713) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CD44 (E7K2Y) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro136 of human CD44 protein. This sequence region is conserved in all isoforms of CD44 reported in Uniprot, with the exception of isoform 2 and isoform 19. Background CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin, and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4). Alternate Names antigen gp90 homing receptor; CD44; CD44 antigen; CD44 antigen (homing function and Indian blood group system); CD44 molecule (Indian blood group); CDw44; CDW44 antigen; cell adhesion molecule; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; Epican; Extracellular matrix receptor III; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; Indian blood group antigen; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH-I; PGP-1; PGP-I; Pgp1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG