CST, 55750S, TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCRdelta. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody (CST #55750) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 61129 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity TRDC/TCR delta (E2E9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRDC/TCRδ protein. This antibody does not cross-react with human TRAC/TCRα constant, TRBC1/TCRβ constant region 1, TRBC2/TCRβ constant region 2, or TRGC1/TCRγ constant region 1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein. Background The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4). Alternate Names B7Z8K6; delta-chain (C-delta region); T cell receptor delta constant; T-cell receptor delta chain C region; TCRD; TRDC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-45 Source/Isotype: Rabbit IgG