CST, 55751S, IgM (E9U8J) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying IGHM. Validated for Western Blotting. Highly specific and rigorously validated in-house, IgM (E9U8J) Rabbit Monoclonal Antibody (Biotinylated) (CST #55751) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IgM (E9U8J) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total IgM heavy chain protein. This antibody reacts with secreted and membrane bound isoforms of IgM heavy chain protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu310 within the constant region of human IgM heavy chain protein. Background Immunoglobulin M (IgM) is one of the five major human immunoglobulin isotypes and is the first antibody to be secreted by plasmablasts in a humoral immune response after exposure to antigen. Structurally, IgM is the largest immunoglobulin and predominantly exists in pentameric form when secreted (1). Alternative splicing of the IgM heavy chain mRNA can generate an alternative form of the antibody, which facilitates its insertion into the plasma membrane of B cells to function in antigen recognition. IgM is the first Ig isotype to appear on the surface of developing B cells and is a major component of the B cell antigen receptor (BCR) signaling complex, which drives B cell survival and proliferation upon antigen-induced ligation (2,3). Research studies have shown that defects in the assembly of the BCR account for the low levels of surface IgM expression in B-chronic lymphocytic leukemia (4,5). Alternate Names AGM1; constant region of heavy chain of IgM; DKFZp686I15196; DKFZp686I15212; FLJ00385; Ig mu chain C region; Ig mu chain C region BOT; Ig mu chain C region GAL; Ig mu chain C region OU; IGHM; IgM; Immunoglobulin heavy constant mu; immunoglobulin mu chain; MGC104996; MGC52291; MU; VH Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG