CST, 55752S, ZNF148 (F5N3O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZNF148. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZNF148 (F5N3O) Rabbit Monoclonal Antibody (CST #55752) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZNF148 (F5N3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZNF148 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu450 of human ZNF148 protein. Background Zinc finger proteins (ZNFs) are one of the largest groups of transcriptional regulatory proteins and consequently are involved in a wide range of cellular processes (1,2). ZNFs have been implicated in transcriptional regulation, ubiquitin-mediated protein degradation, signal transduction, actin targeting, DNA repair, as well as cell proliferation, migration, and apoptosis (2). ZNFs have been shown to interact with DNA, RNA, poly-ADP-ribose (PAR), and other proteins (1,2). ZNF148, also known as ZBP89, was first discovered and characterized as a C2H2 family transcription factor (1) and has been primarily studied for its role in cancer cell biology. Several tumor types, such as breast, liver, skin, brain, and gastric cancers, all exhibit increased ZNF148 expression versus normal tissue, suggesting an oncogenic function (3-9). Conversely, ZNF148 has also been shown to act as a tumor suppressor in colorectal cancers by repressing cell proliferation and inducing apoptosis (4,5). The likely reason for these dual roles for ZNF148 is that it has two functionally distinct alternative splicing isoforms: one encoding the full-length protein, and one that lacks the N-terminal domain (1,2). These two alternatively spliced isoforms have been shown to act antagonistically to each other (2). This dual regulatory role of ZNF148, along with its interaction with multiple proteins that facilitate critical aspects of tumor biology, make it a key protein of interest for cancer biologists. Alternate Names BERF-1; BFCOL1; CACCC box-binding protein; CLL-associated antigen KW-10; GDACCF; HT-BETA; pHZ-52; Transcription factor ZBP-89; ZBP-89; ZBP89; ZFP148; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148; ZNF148 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG