CST, 55829S, CMTM6 Antibody

Polyclonal Antibody for studying CMTM6 mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CMTM6 Antibody (CST #55829) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CMTM6 Antibody recognizes endogenous levels of total mouse CMTM6 protein. This antibody cross-reacts with an unidentified protein of 35 kDa, 55 kDa, and 75 kDa in some cell extracts. Species Reactivity: Mouse Source / Purification Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of mouse CMTM6 protein. Antibodies are purified by peptide affinity chromatography. Background CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) is a member of the chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (1). CMTM6 stabilizes plasma membrane expression of PD-L1, an immune inhibitory ligand critical for immune tolerance to self and anti-tumor immunity (2,3). CMTM6 associates with PD-L1 at recycling endosomes, where it protects PD-L1 from being targeted for lysosomal degradation by preventing STUB1-mediated PD-L1 ubiquitination (2,3). CMTM6 may stabilize PD-L1 expression on antigen presenting cells and potentiate inhibitory signaling by PD-1 on T cells, triggering T cell inhibition and exhaustion. CMTM6 has also been shown to interact with CD58, ARG1, ENO1, and TMPO (2). Due to the role of CMTM6 in regulating the immune system, it is being investigated as an immunotherapeutic target for the treatment of cancer. Alternate Names 2810051A14Rik; AA536733; chemokine-like factor super family 6; chemokine-like factor superfamily 6; Chemokine-like factor superfamily member 6; CKLF-like MARVEL transmembrane domain containing 6; CKLF-like MARVEL transmembrane domain-containing protein 6; CKLF6; Cklfs; Cklfsf6; Cmtm6; Q9CZ69 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit