CST, 5585T, Tyro3 (D38C6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tyro3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Tyro3 (D38C6) Rabbit Monoclonal Antibody (CST #5585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 64590 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Tyro3 (D38C6) Rabbit Monoclonal Antibody detects endogeneous levels of total Tyro3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr874 of human Tyro3 protein. Background Tyro3 is a receptor tyrosine kinase belonging to the TAM subfamily (Tyro3, Axl, and Mer). All three members have similar domain structures composed of an extracellular region with 2 Ig-like domains, followed by 2 FNII-like domains, a single transmembrane region, and a cytoplasmic tyrosine kinase domain (1). The natural ligand for Tyro3, as well as Axl and Mer, is GAS6 (growth arrest specific gene 6) (1,2). Expression pattern and target knockout data indicate an important role of Tyro3 in apoptotic cell phagocytosis of dendritic cells and macrophages (3), NK cell differentiation (4), reproductive neuron survival and migration (5), osteoclast stimulation (6,7), as well as cortical and hippocampal neuron function (8). Both MAPK and PI3K pathways have been suggested as downstream targets of Tyro3 activation (7,8). Tyro3 has also been shown to be correlated to melanoma tumorigenesis, likely through its regulatory role in the expression of oncogenic microphthalmia-associated transcription factor (MITF) (9). Alternate Names Brt; BYK; DTK; Etk-2; FLJ16467; Rek; RSE; SKY; Tif; TYRO3; TYRO3 protein tyrosine kinase; Tyro3 protein tyrosine kinase (sea-related receptor tyrosine kinase); Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110, 130 Source/Isotype: Rabbit IgG