CST, 55883S, Villin-1 (CWWB1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Villin1. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Villin-1 (CWWB1) Mouse Monoclonal Antibody (CST #55883) is ready to ship. Product Usage Information IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Villin-1 (CWWB1) Mouse Monoclonal Antibody recognizes endogenous levels of villin-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein containing amino acids 5-209 of mature villin-1 protein. Background Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81, and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin-1 function appears to be involved in progressive cholestasis and hepatic failure (5). Alternate Names D2S1471; VIL; VIL1; VILI; Villin; villin 1; Villin-1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa