CST, 5589S, Rab11 (D4F5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rab11A/Rab11B. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rab11 (D4F5) Rabbit Monoclonal Antibody (CST #5589) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73006 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rab11 (D4F5) Rabbit Monoclonal Antibody detects endogeneous levels of Rab11 protein, including isoforms Rab11a and Rab11b. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Rab11 protein. Background Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6). Alternate Names GTP-binding protein YPT3; H-YPT3; MGC133246; MGC1490; NDAGSCW; RAB 11A, member oncogene family; Rab-11; RAB11; RAB11A; RAB11A, member RAS oncogene family; RAB11B; RAB11B, member of RAS oncogene family; RAB11B, member RAS oncogene family; Ras-related protein Rab-11A; Ras-related protein Rab-11B; RB11A; RB11B; YL8; YPT3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG