CST, 55901SF, Cofilin (D3F9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying cofilin 1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),ELISA+. Highly specific and rigorously validated in-house, Cofilin (D3F9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #55901) is ready to ship. Product Usage Information This product is the carrier free version of product #5175. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5175 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cofilin (D3F9) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total cofilin1 protein. Species Reactivity: Human, Mouse, Rat, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein. Background Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). Alternate Names 18 kDa phosphoprotein; CFL; CFL1; COF1; cofilin; cofilin 1; cofilin 1 (non-muscle); Cofilin-1; Cofilin, non-muscle isoform; epididymis secretory protein Li 15; HEL-S-15; p18 Specification REACTIVITY: H M R Mk Dg SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG