CST, 55920S, mGluR5 (D6E7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying mGluR5. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, mGluR5 (D6E7B) Rabbit Monoclonal Antibody (CST #55920) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity mGluR5 (D6E7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total mGluR5 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human mGluR5 protein. Background mGluR5, a metabotropic glutamate receptor, is a class C G protein-coupled receptor that signals through the Gaq/11-PLC-inositol 1,4,5 triphosphate pathway (1). mGluR5 is comprised of a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular domain. Glutamate binding to mGluR5 leads to an increase in intracellular calcium levels and stimulation of PKC activity (2). In neurons, mGluR5 is found in the post-synapse, in a complex with NMDA receptors, PSD-95, SHANK, and Homer (3). mGluR5 is also expressed in microglia and astrocytes (4). Neuronal mGluR5 has been shown to interact with amyloid beta oligomers, and mGluR5 antagonists exhibit neuroprotective effects (5) placing mGluR5 as a potential therapeutic target for Alzheimer's disease. In glial cells, mGluR5 appears to play an anti-inflammatory role by negatively regulating the release of inflammatory factors (6). Alternate Names glutamate metabotropic receptor 5; glutamate receptor, metabotropic 5; GPRC1E; GRM5; Metabotropic glutamate receptor 5; mGlu5; MGLUR5; PPP1R86; protein phosphatase 1, regulatory subunit 86 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 300, 150 Source/Isotype: Rabbit IgG