CST, 55929SF, Stat5 (D2O6Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying STAT5A/STAT5B. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Stat5 (D2O6Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #55929) is ready to ship. Product Usage Information This product is the carrier free version of product #94205. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 94205 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Stat5 (D2O6Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Stat5 protein. This antibody recognizes both alpha and beta isoforms of Stat5 (Stat5a and Stat5b). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human Stat5 protein. Background Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Alternate Names epididymis secretory sperm binding protein; GHISID2; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5A; STA5B; STAT5; STAT5A; STAT5B; transcription factor STAT5B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG