CST, 55935S, SH3BP4 (D3D9I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SH3BP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SH3BP4 (D3D9I) Rabbit Monoclonal Antibody (CST #55935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SH3BP4 (D3D9I) Rabbit Monoclonal Antibody recognizes endogenous levels of total SH3BP4 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human SH3BP4 protein. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by enviromental factors such as energy levels, growth factors, and amino acids (3, 4). The GTPases RagA, RagB, RagC, and RagD mediate amino acid signaling to activate mTORC1 (1, 2). SH3BP4 (SH3 domain-binding protein 4) binds to the inactive Rag GTPase complex during amino acid starvation and prevents the association of Rag GTPase complex with mTORC1 resulting in the suppression of mTORC1 activation and cell growth (5). Alternate Names BOG25; EH-binding protein 10; EHB10; SH3 domain binding protein 4; SH3 domain-binding protein 4; SH3-domain binding protein 4; SH3B4; SH3BP4; transferrin receptor trafficking protein; Transferrin receptor-trafficking protein; TTP Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG