CST, 5593S, Troponin T (Cardiac) Antibody

Polyclonal Antibody for studying TNNT2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Troponin T (Cardiac) Antibody (CST #5593) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro69 of human cardiac Troponin T protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca concentration. Troponin consists of three subunits: the Ca -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca sensitivity by reducing the Ca binding affinity of TnC (1-3). The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury. Alternate Names Cardiac muscle troponin T; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; RCM3; TNNT2; TnTc; troponin T type 2 (cardiac); troponin T type 2, cardiac; Troponin T, cardiac muscle; troponin T2, cardiac; troponin T2, cardiac type; truncated cardiac troponin T Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit