CST, 55959T, TOM40 (E6Q3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TOMM40. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TOM40 (E6Q3Z) Rabbit Monoclonal Antibody (CST #55959) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TOM40 (E6Q3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of both isoforms of total TOM40 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala146 of human TOM40 protein. Background Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1). TOM20 is localized in the outer mitochondrial membrane and initially recognizes precursors with a presequence to facilitate protein import across the outer mitochondrial membrane (2). In a sequential process, recognition of the presequence by TOM20 is followed by tethering of the presequence to the TOM40 protein complex for efficient protein import (3). Alternate Names C19orf1; D19S1177E; Mitochondrial import receptor subunit TOM40 homolog; mitochondrial outer membrane protein; p38.5; PER-EC1; PEREC1; Protein Haymaker; TOM40; TOMM40; Translocase of outer membrane 40 kDa subunit homolog; translocase of outer mitochondrial membrane 40; translocase of outer mitochondrial membrane 40 homolog; translocase of outer mitochondrial membrane 40 homolog (yeast) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 39, 41 Source/Isotype: Rabbit IgG