CST, 5595S, SGK2 Antibody

Polyclonal Antibody for studying SGK2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SGK2 Antibody (CST #5595) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SGK2 Antibody detects endogenous levels of total SGK2 protein Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe386 of human SGK2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). Alternate Names dJ138B7.2; H-SGK2; Serine/threonine-protein kinase Sgk2; serum/glucocorticoid regulated kinase 2; Serum/glucocorticoid-regulated kinase 2; SGK2; SGK2 iso1; SGK2, serine/threonine kinase 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit