CST, 55962S, HOXD9 (E3F2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HOXD9. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HOXD9 (E3F2T) Rabbit Monoclonal Antibody (CST #55962) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity HOXD9 (E3F2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOXD9 protein. This antibody does not cross-react with HOXA9, HOXB9 or HOXC9 proteins. This antibody also weakly recognizes non-specific bands of unknown origin at 55 and 70 kDa in some cell lines. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly159 of human HOXD9 protein. Background Homeobox protein Hox-D9 (HOXD9) is a sequence-specific transcription factor that is part of a developmental regulatory program that provides cells with specific positional identities on the anterior-posterior axis. HOXD9 is developmentally expressed in structures of either mesodermal or neuro-ectodermal origin, such as developing limbs, gonads, and the central nervous system (1-6). HOXD9 plays a critical role in regulation of limb development, neuronal development, and development of mammary glands and gonads in many organisms (1-6). The HOXD9 gene promoter is found to be hypermethylated and silenced in multiple types of cancer, including breast cancer, melanoma brain metastases, and cholangiocarcinomas (7-9). In addition, HOXD9 expression is increased in other types of cancer, including human glioblastomas and astrocytomas, where expression appears to drive growth of the tumors (10). Alternate Names homeo box D9; homeobox D9; Homeobox protein Hox-4C; Homeobox protein Hox-5.2; Homeobox protein Hox-D9; Hox-4.3; Hox-4.3, mouse, homolog of; Hox-5.2; HOX4; HOX4C; HOXD9; HXD9 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG