CST, 5601S, beta-IG-H3 (D31B8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying betaIG-H3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, beta-IG-H3 (D31B8) Rabbit Monoclonal Antibody (CST #5601) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity beta-IG-H3 (D31B8) Rabbit Monoclonal Antibody detects endogenous levels of total βIG-H3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human βIG-H3 protein. Background βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). Alternate Names Beta ig-h3; beta-IG-H3; beta-IGH3; betaIG-H3; BGH3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; transforming growth factor beta induced; transforming growth factor beta-induced 68kDa; Transforming growth factor-beta-induced protein ig-h3; transforming growth factor, beta-induced, 68kD; transforming growth factor, beta-induced, 68kDa Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG