CST, 56075T, MERS-CoV Membrane Protein (E2Z4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Membrane Protein MERS-CoV. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MERS-CoV Membrane Protein (E2Z4Q) Rabbit Monoclonal Antibody (CST #56075) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MERS-CoV Membrane Protein (E2Z4Q) Rabbit Monoclonal Antibody recognizes transfected levels of total MERS-CoV membrane protein. This antibody does not cross-react with membrane proteins from SARS coronaviruses (CoV-1, CoV-2). Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln184 of MERS-CoV membrane protein. Background Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus; similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins required for viral replication (3). The MERS membrane protein, like that of other betacoronaviruses, is an integral membrane protein and the most abundant of the structural proteins in a virus particle. Numerous studies have shown that the membrane protein of betacoronaviruses is more than just a structural protein; it plays multiple functional roles in orchestrating viral replication following host infection (4-7). Alternate Names E1 glycoprotein; M protein; Matrix glycoprotein; Membrane glycoprotein; membrane protein; MERS Membrane Protein; MERS-CoV Membrane protein; VME1_CVEMC Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 20-30 Source/Isotype: Rabbit IgG