CST, 56082S, CD14 (D7A2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD14. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD14 (D7A2T) Rabbit Monoclonal Antibody (CST #56082) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD14 (D7A2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD14 protein. This antibody cross-reacts with a 22 kDa protein of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro319 of human CD14 protein. Background CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5). Alternate Names CD14; CD14 antigen; CD14 molecule; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-60 Source/Isotype: Rabbit IgG