CST, 56085S, ARL8B Antibody

Polyclonal Antibody for studying ARL8B. Validated for Western Blotting. Highly specific and rigorously validated in-house, ARL8B Antibody (CST #56085) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ARL8B Antibody recognizes endogenous levels of total ARL8B protein. This antibody does not cross-react with ARL8A. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala137 of human ARL8B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The small GTPases of the Arf and Rab families have critical roles in cytoskeletal organization and membrane/vesicular transport (1,2). The related ADP-ribosylation factor-like (ARL) family member ARL8, including two paralogs ARL8A and ARL8B, has emerged as an important regulator of lysosomal transport to the cell periphery (3,4). Lysosome positioning is a critical determinant of a number of physiological processes including plasma membrane repair, cell migration, antigen presentation, and response to nutrient availability. Membrane association of ARL8B is regulated by the BLOC-one related complex (BORC), a multi-subunit complex under the control of the mTOR pathway (5,6). Effectors of GTP-bound ARL8B include PLEKHM2/SKIP and the Vps41 subunit of the HOPS complex (7). Studies have found that ARL8B may be a critical factor for NK-mediated cytotoxicity by driving polarization of lytic granules toward the immune synapse (8). In addition, ARL8B has been shown to act as a negative regulator of autophagy (9). Knockdown of ARL8B resulted in increased fusion of autophagosomes with lysosomes. Furthermore, ARL8B depleted cells had reduced levels of pathogenic substrates associated with neurodegenerative diseases. Alternate Names ADP ribosylation factor like GTPase 8B; ADP-ribosylation factor-like 10C; ADP-ribosylation factor-like 8B; ADP-ribosylation factor-like protein 10C; ADP-ribosylation factor-like protein 8B; ARL10C; ARL8B; FLJ10702; GIE1; Novel small G protein indispensable for equal chromosome segregation 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit