CST, 56086SF, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IFIT1. Validated for WB,IHC,IHC,F. Highly specific and rigorously validated in-house, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56086) is ready to ship. Product Usage Information This product is the carrier free version of product #14769. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14769 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IFIT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein. Background IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by type I interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5' triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor 3 (eIF3) (3). Alternate Names C56; G10P1; GARG-16; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; interferon induced protein with tetratricopeptide repeats 1; Interferon-induced 56 kDa protein; interferon-induced protein 56; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561; Interferon, alpha-inducible protein (MW 56kD); ISG56; P56; RNM561 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG