CST, 5617T, Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARHGAP42 (Tyr376) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit Monoclonal Antibody (CST #5617) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit Monoclonal Antibody detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein. Background ARHGAP family members activate Rho GTPase proteins (1). Mutations of ARHGAP family genes lead to carcinogenesis through the dysregulation of Rho/Rac/Cdc42-like GTPases (2). Little is known about the biological function of ARHGAP42, a member of the ARHGAP family. ARHGAP42 contains a RhoGAP domain in the middle of its sequence, an SH3 binding domain at the C-terminal end of the protein sequence, as well as a BAR domain and a PH domain in the N-terminal region of the sequence, which indicate that the protein may function as GTPase activator and cytoskeletal adaptor. Alternate Names AD031; ARHGAP42; FLJ32810; GRAF3; KIAA0621; RHG42; Rho GTPase activating protein 42; rho GTPase-activating protein 10-like; Rho GTPase-activating protein 42; Rho GTPase-activating protein FLJ32810; Rho-type GTPase-activating protein 42; Rho-type GTPase-activating protein FLJ32810; TMEM133; transmembrane protein 133; YK021 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG