CST, 56191S, ELOF1 (F6D3C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ELOF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ELOF1 (F6D3C) Rabbit Monoclonal Antibody (CST #56191) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ELOF1 (F6D3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELOF1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val37 of human ELOF1 protein. Background DNA lesions in the template strand of actively transcribed genes result in genome-wide transcriptional arrest. The transcription-coupled nucleotide excision repair (TC-NER) pathway efficiently eliminates transcription-blocking DNA lesions (TBLs) (1). ELOF1 is a component of the transcriptional elongating Pol II complex (2). The protein ensures high-speed elongation processivity during mRNA transcription on the DNA template (3). ELOF1 is an important player of the TC-NER-complex (4). ELOF1 facilitates Pol II ubiquitylation and ensures correct TC-NER-complex assembly upon DNA damage (5). The protein promotes UV-stimulated scaffold protein A (UVSSA) and lesion-stalled Pol II binding, resulting in subsequent transcription factor IIH (TFIIH) recruitment, which is crucial in the full incision complex assembly to excise the TBL and restart transcription (6). Alternate Names ELF1; ELF1 homolog, elongation factor 1; ELOF1; elongation factor 1 homolog; elongation factor 1 homolog (ELF1, S. cerevisiae); elongation factor 1 homolog (S. cerevisiae); Transcription elongation factor 1 homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG