CST, 5622S, Phospho-Semaphorin 4B (Ser825) Antibody

Polyclonal Antibody for studying Semaphorin 4B (Ser830) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Semaphorin 4B (Ser825) Antibody (CST #5622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Semaphorin 4B (Ser825) Antibody detects endogenous levels of semaphorin 4B protein only when phosphorylated at Ser825. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser825 of human semaphorin 4B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The semaphorin family of proteins is involved in axon guidance, cell migration, angiogenesis, and immune response. Plexins and neuropilins bind with high affinity to semaphorins to mediate their functions. Semaphorins are divided into seven classes of secreted or membrane-bound proteins. Members of Class 4 semaphorins include 4A through 4G; semaphorin 4B is a membrane-associated protein (1,2). Semaphorin 4B binds to the CLCP1 receptor and regulates cell motility (3). Furthermore, semaphorin 4B, like many other semaphorins, has a PDZ domain-binding motif at the carboxy terminus that interacts with PSD95 and localizes to the post-synaptic membrane (4). The phosphorylation site at Ser825 was identified at Cell Signaling Technology using PTMScan Technology, a proprietary proteomic method for post-translational modification site discovery (5). Alternate Names KIAA1745; MGC131831; SEM4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B; SEMA4B; SEMA4B iso3; SEMAC; semaphorin 4B; Semaphorin-4B; Semaphorin-C; SemC Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 105 SOURCE: Rabbit