CST, 56298S, LDHB (E8J8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LDH-B. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, LDHB (E8J8T) Rabbit Monoclonal Antibody (CST #56298) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LDHB (E8J8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total LDHB protein. This antibody does not cross-react with LDHA or LDHC protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala13 of human LDHB protein. Background Lactate dehydrogenase (LDH) catalyzes the reversible conversion between pyruvate and lactate. LDH is a tetramer composed of various combinations of LDHA subunit and LDHB subunit to form five different isozymes. LDHA has a higher affinity for pyruvate and preferentially catalyzes the conversion of pyruvate to lactate. LDHA levels are upregulated in many cancers. On the other hand, LDHB has a higher affinity for lactate and preferentially catalyzes the conversion of lactate to pyruvate, enabling cells to use lactate as a nutrient (1-3). Studies show that LDHA/LDHB deficiency suppresses glycolysis and ATP production, inhibiting STING signaling and antitumor immune responses mediated by dendritic cells (4). In addition, acetylation of LDHB inhibits its activity, reduces hepatic lactate clearance, and promotes the progression of non-alcoholic fatty liver disease (NAFLD) (5). Alternate Names epididymis secretory protein Li 281; HEL-S-281; L-lactate dehydrogenase B chain; lactate dehydrogenase B; lactate dehydrogenase H chain; LDH heart subunit; LDH-B; LDH-H; LDHB; LDHBD; Renal carcinoma antigen NY-REN-46; testicular secretory protein Li 25; TRG-5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG