CST, 5629S, RXR gamma Antibody

Polyclonal Antibody for studying RXRgamma. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RXR gamma Antibody (CST #5629) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RXR gamma Antibody recognizes endogenous levels of total RXRγ protein. This antibody does not cross-react with either RXRα or RXRβ. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRγ protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The human retinoid X receptors (RXRs) are encoded by three distinct genes ( α, β, and γ) and bind selectively and with high affinity to the vitamin A derivative, 9- retinoic acid. RXRs are type-II nuclear hormone receptors that are primarily localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to -acting response elements in the promoter/enhancer region of target genes (2). RXRγ expression, unlike that of RXRα and RXRβ, is restricted largely to cardiac muscle, skeletal muscle, thyroid gland, and thyrotrope cells of the anterior pititary gland (3-6). It is posited that RXRγ plays a pivitol role in the transcriptional control of skeletal muscle differentiation as RXRγ has been found to be directly associated with the promoter regions of and (7-10). RXRγ is also involved in the transcriptional control of the hypothalamic-pituitary-thyroid axis through repression of the β promoter (11-13). Alternate Names NR2B3; Nuclear receptor subfamily 2 group B member 3; Retinoic acid receptor RXR-gamma; Retinoid X receptor gamma; retinoid X receptor-gamma; retinoid X receptor, gamma; RXRC; RXRG Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit