CST, 56308S, PADI4 (F1G7E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PADI4. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PADI4 (F1G7E) Rabbit Monoclonal Antibody (CST #56308) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity PADI4 (F1G7E) Rabbit Monoclonal Antibody recognizes endogenous levels of total PADI4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human PADI4 protein. Background Peptidyl arginine deiminase (PAD) proteins are a family of Ca -dependent enzymes that catalyze the post-translational conversion of arginine to citrulline. There are currently five known PAD isozymes in humans, referred to as PADI1-4 and PADI6 (1). PADI4 is a nuclear member of the PAD family and is responsible for deiminating R2, R8, R17, and R26 on the histone H3 (2-4). Deimination of histone residues prevents CARM1-mediated arginine methylation and results in transcriptional repression (2,3). Expression of PADI4 is increased during myeloid differentiation and various polymorphisms have been implicated in rheumatoid arthritis (5-8). PADI4 can play a role in apoptosis and cell cycle arrest upon DNA damage, as it is recruited to the p21 promoter by p53 and also regulates a subset of p53 target genes (9,10). Hypercitrullination of histones by PADI4 contributes to chromatin decondensation and the formation of neutrophil extracellular traps (NETs) to combat bacterial infection in a process called NETosis (11,12). Inhibition of PADI4 reduces NET formation, making it a potential therapeutic target (13). Alternate Names HL-60 PAD; PAD; PAD4; PADI-H protein; PADI4; PADI5; PDI4; PDI5; peptidyl arginine deiminase 4; peptidyl arginine deiminase, type IV; peptidyl arginine deiminase, type V; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV; Protein-arginine deiminase type-4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG