CST, 56310S, SLMAP (E5F4Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLMAP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SLMAP (E5F4Y) Rabbit Monoclonal Antibody (CST #56310) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SLMAP (E5F4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLMAP protein. Based on antigen sequence, the antibody is predicted to detect isoforms 1-4 and isoform 6 of SLMAP. A band of unknown origin is detected at ~60 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr462 of human SLMAP protein. Background Sarcolemmal membrane-associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process (1). It is also a component of a conserved striatin-interacting phosphatase and kinase complex (STRIPAK) which participates in a variety of cellular processes, including signaling, cell cycle control, cell migration, Golgi assembly, and apoptosis (2-4). There are several isoforms of SLMAP as a result of alternative splicing; shorter isoforms exhibit high expression in cardiac muscle, whereas the longer isoforms are expressed more ubiquitously, but at lower levels (5). As a cardiac membrane protein, SLMAP plays an important role in excitation-contraction coupling and the adrenergic response, and has been identified as a novel regulator of cardiac function (6-8). In support of that function, mutations in the gene are associated with Brugada syndrome, a cardiac channelopathy (9). Deregulation of SLMAP expression has also been implicated in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes (10). Additionally, a novel SLMAP-ALK fusion has been identified in a patient with non-small cell lung cancer (11). Alternate Names FLJ42206; KIAA1601; MGC138760; MGC138761; sarcolemma associated protein; Sarcolemmal membrane-associated protein; Sarcolemmal-associated protein; SLAP; SLMAP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90-95 Source/Isotype: Rabbit IgG