CST, 5638S, Phospho-TNK1 (Tyr277) (D46E7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tnk1 (Tyr277) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TNK1 (Tyr277) (D46E7) Rabbit Monoclonal Antibody (CST #5638) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TNK1 (Tyr277) (D46E7) Rabbit Monoclonal Antibody detects endogenous levels of TNK1 protein only when the amplified gene product is phosphorylated at Tyr277. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr277 of human TNK1 protein. Background Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3). Phosphorylation of TNK1 on Tyr277 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery (4) and also reported independently in another publication using MS technology (5). Phosphorylation of TNK1 at Tyr277 was observed in select carcinoma cell lines and in tumors. A constitutively active, 58 kDa truncated TNK1 kinase resulting from fusion between the TNK1 and C17orf61 genes is seen in some cells (5). For additional information, visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org. Alternate Names CD38 negative kinase 1; KOS1; MGC46193; Non-receptor tyrosine-protein kinase TNK1; TNK1; tyrosine kinase non receptor 1; tyrosine kinase, non-receptor, 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72 TNK1, 58 TNK1-C17orf61 Source/Isotype: Rabbit IgG