CST, 5641S, Ubc12 (D13D7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBC12. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Ubc12 (D13D7) Rabbit Monoclonal Antibody (CST #5641) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Ubc12 (D13D7) Rabbit Monoclonal Antibody detects endogeneous levels of total Ubc12 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human Ubc12 protein. Background Similar to ubiquitin, NEDD8 is covalently linked to target proteins through an enzymatic cascade composed of NEDD8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for NEDD8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 are cullin-based RING E3 ligases. Neddylation of cullin isoforms activates the related ubiquitin E3 complex by promoting its interaction with a cognate ubiquitin-E2 ligase (6-7). Neddylation of Cul-1 complexes containing βTrCP and SKP2 has been shown to be required for controlling the stability of important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11). Alternate Names epididymis secretory sperm binding protein; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBE2M; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2 M; ubiquitin conjugating enzyme E2M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG