CST, 56426S, PRAME (E7I1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRAME. Validated for WB,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, PRAME (E7I1B) Rabbit Monoclonal Antibody (CST #56426) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 19600 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PRAME (E7I1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRAME protein. Non-specific non-nuclear staining was observed in smooth muscle. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly163 of human PRAME protein. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). PRAME (preferentially expressed antigen in melanoma) is a cancer/testis antigen not normally expressed in any tissues except testis, but is upregulated in tumors. PRAME is expressed in melanoma cells and is recognized by cytolytic T-cells (4). It is also upregulated in other diseases, such as synovial sarcoma (5), NSCLC (6), and breast cancer, where it is thought to contribute to tumorigenesis and metastasis (7). PRAME is also highly expressed in liquid tumors such as AML (8) and can be predictive of clinical outcome in some circumstances (9). PRAME and other cancer/testis antigens are currently being pursued as novel immunotherapy targets and diagnostic biomarkers (10). Alternate Names cancer/testis antigen 130; CT130; MAPE; Melanoma antigen preferentially expressed in tumors; OIP-4; OIP4; Opa-interacting protein 4; Opa-interacting protein OIP4; PRAME; PRAME nuclear receptor transcriptional regulator; preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG