CST, 5642S, Phospho-SGK3 (Thr320) (D30E6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SGK3 (Thr320) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SGK3 (Thr320) (D30E6) Rabbit Monoclonal Antibody (CST #5642) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-SGK3 (Thr320) (D30E6) Rabbit Monoclonal Antibody detects overexpressed levels of SGK3 protein only when phosphorylated at Thr320. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human SGK3 protein. Background Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). SGK3 has been shown to be a downstream signaling molecule in the PI3K pathway. Its activation and phosphorylation at Thr320 by PDK1 may be an Akt-independent manner of signaling in cancer (8). Alternate Names CISK; cytokine-independent survival kinase; DKFZp781N0293; Serine/threonine-protein kinase Sgk3; serum/glucocorticoid regulated kinase 3; serum/glucocorticoid regulated kinase family member 3; serum/glucocorticoid regulated kinase family, member 3; serum/glucocorticoid regulated kinase-like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK2; SGK3; SGKL Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 62 Source/Isotype: Rabbit IgG